The change rule of biogenic amines during the processing of Sanchuan ham.

With Sanchuan ham made by traditional fermentation process as raw material, the mass concentration of 8 main biogenic amines in the curing, drying and fermentation stages of Sanchuan ham was determined by HPLC, and the change rule of biogenic amines in the processing of Sanchuan ham was studied. The results showed that: 1) a total of 7 kinds of biogenic amines were detected, which were phenethylamine, resinamine, saprolamine, histamine, tyramine, ispermine and spermine. Tryptamine was not detected out. Among them, sapromine and spermine existed in the whole process of Sanchuan ham. The cadaverine mass concentration was the highest in 7 species, followed by spermine. The cadaverine mass concentration continued to increase during the processing of Sanchuan ham, and reached the maximum value in the late stage of fermentation, which was (69.24 ±12.54) mg/kg, but began to decrease significantly in the late stage of fermentation. The mass concentration of spermidine and spermine were stable, and the mass concentration of spermidine was low. Phenethylamine was detected only in the middle of curing. The mass concentration of histamine was low, and the highest mass concentration was only (22.52 ±5. 25) mg/kg, which was relatively safe. 2) during the whole process of Sanchuan ham, the total biogenic amine mass concentration first increased, then stabilized and then decreased: the total biogenic amine mass concentration in fresh Sanchuan ham was the lowest, which was (5.84 ± 1.30) mg/kg, and the total biogenic amine mass concentration increased significantly in the middle and late stage of curing ( P < 0. 05 ) . During the drying period, the increase of total biogenic amine mass concentration was not significant ( P > 0. 05 ) . In the late stage of fermentation, the mass concentration of biogenic amine was the highest, which was (158.08 ±31.35) mg/kg. [ABSTRACT FROM AUTHOR]