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Entropy-driven catalytic reaction-induced hairpin structure switching for fluorometric detection of uranyl ions.
- Source :
-
Microchimica Acta . Sep2019, Vol. 186 Issue 9, pN.PAG-N.PAG. 1p. - Publication Year :
- 2019
-
Abstract
- An ultra-sensitive and "turn-on" method is demonstrated for the determination of uranyl ion. The assay is based on hairpin-to-DNAzyme structure switching that is induced by an entropy-driven catalytic reaction. An UO22+-specific DNAzyme is cleaved by UO22+ to produce a DNA fragment. This fragment initiates the entropy-driven catalytic reaction to produce a large number of a sequence "R". The sequence R initiates the circular cleavage of FAM-labeled hairpins by switching the hairpin to Mg2+-specific DNAzyme structure. This causes the recovery of green fluorescence. The method works in the 20 pM to 800 pM concentration range and the limit of detection is 4 pM. [ABSTRACT FROM AUTHOR]
- Subjects :
- *DETECTION limit
*IONS
Subjects
Details
- Language :
- English
- ISSN :
- 00263672
- Volume :
- 186
- Issue :
- 9
- Database :
- Academic Search Index
- Journal :
- Microchimica Acta
- Publication Type :
- Academic Journal
- Accession number :
- 138578829
- Full Text :
- https://doi.org/10.1007/s00604-019-3767-0