Back to Search
Start Over
Molecular cloning and nucleotide sequence of cDNA specific for rat ribosomal protein L5.
- Source :
-
European Journal of Biochemistry . 10/1/87, Vol. 168 Issue 1, p83-87. 5p. - Publication Year :
- 1987
-
Abstract
- cDNA clones specific for rat ribosomal protein L5, which is the protein moiety of 5S RNA protein particles, were isolated by using a mixture of tetradecamer deoxyribonucleotide probes reflecting the amino acid sequence of the N-terminal portion of L5. A cDNA clone (pRL5-1) lacking the 3′-terminal region of L5 was isolated by hybrid-selected translation and shown to be L5-specific by the identity of the deduced amino acid sequence and that of the N-terminus of protein L5. pRL5-1 was then used to select another clone (pRL5-2) by colony hybridization which contained the entire coding region, 3′-non-coding region and poly(A) addition signal. The nucleotide sequence of L5-specific cDNA was determined by using these two clones. It contained a total of 1069 base pairs: 123 base pairs in the 5′-non-coding region, 894 base pairs in the coding region and 52 base pairs in the 3′-non-coding region. The poly(A) addition signal appeared at the 33rd nucleotide after the termination co- don. The amino acid sequence deduced from this nucleotide sequence consists of 296 amino acids. The homology of amino acid sequence between L5 and other 5S RNA-binding proteins is discussed. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00142956
- Volume :
- 168
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- European Journal of Biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 13857818
- Full Text :
- https://doi.org/10.1111/j.1432-1033.1987.tb13390.x