Preparation of hydroxypropyl cellulose-poly (2-Methacryloyloxyethyl phosphorylcholine) coating for the analysis of proteins by capillary electrophoresis.

We reported a new method for preparing coated capillaries in this paper. First, we combined hydroxypropyl cellulose (HPC) and 2-Methacryloyloxyethyl phosphorylcholine (MPC) to form hydroxypropyl cellulose-poly (2-Methacryloyloxyethyl phosphorylcholine) (HPC-PMPC) by atom transfer radical polymerization (ATRP). Then the photosensitive polymer diazoresin (DR) was used as a linker to the inner wall of the capillary, and finally exposed to ultraviolet light to form a stable covalent bond coating. Protein adsorption on the inner surface of capillary was effectively inhibited. The cytochrome c (Cyt-c), lysozyme (Lys), ribonuclease A (RNase A) and bovine serum albumin (BSA) were separated obviously using capillary electrophoresis (CE) within 15 minutes and showed good stability and repeatability. [ABSTRACT FROM AUTHOR]