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Dissection of loci conferring resistance to stripe rust in Chinese wheat landraces from the middle and lower reaches of the Yangtze River via genome-wide association study.
- Source :
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Plant Science . Oct2019, Vol. 287, pN.PAG-N.PAG. 1p. - Publication Year :
- 2019
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Abstract
- • Chinese wheat landraces were assessed for stripe rust resistance at the adult-plant stage. • Performed a genome-wide association study to APR stripe rust using DArT array and SSR markers. • Identified eight potential novel APR loci for wheat stripe rust. • Haplotype analysis revealed that accessions carrying APR loci showed enhanced degrees of resistance. Stripe rust (Yr), caused by the fungal pathogen Puccinia striiformis f. sp. tritici , is a devastating foliar disease of wheat in China. Chinese wheat landraces originating from the middle and lower reaches of the Yangtze River are potential stripe-rust resistance resources. To identify APR genes for stripe-rust resistance, a panel of 188 accessions derived from the middle and lower reaches of the Yangtze River were inoculated with a mixture of Chinese P. striiformis f. sp. tritici races and resistance evaluated under field conditions in five environments at adult-plant stages. Seventy-three accessions showed degrees of stable resistance. Combining phenotypic datasets from multiple field experiments with high-quality Diversity Arrays Technology and simple sequence repeat markers, we detected 21 marker-trait associations spanning 18 quantitative trait loci (QTLs) on chromosomes 1B, 2A, 2B, 3B, 4A, 5A, 5B, 6B, and 6D, respectively. Single QTLs explained 9.67% to 16.14% of the observed phenotypic variation. Nine QTLs co-localized with previously reported Yr genes or genomic regions. The remaining QTLs were potential novel loci associated with adult-stage stripe-rust resistance. Two novel QTLs, QYr.sicau-3B.2 and QYr.sicau-5B.3 , located on chromosomes 3B and 5B significantly explained 16.14% and 11.16% of the phenotypic variation, respectively. Haplotype analysis revealed that accessions carrying APR variants or their combinations showed enhanced degrees of resistance. The potentially novel loci or genomic regions associated with adult-stage resistance may be useful to improve stripe-rust resistance in current wheat cultivars and for future isolation of stripe-rust resistance genes. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 01689452
- Volume :
- 287
- Database :
- Academic Search Index
- Journal :
- Plant Science
- Publication Type :
- Academic Journal
- Accession number :
- 138389404
- Full Text :
- https://doi.org/10.1016/j.plantsci.2019.110204