电针对大鼠心肺复苏后脑损伤的保护作用 及对海马炎性因子表达的影响.

Objective: To investigate the effect of electroacupuncture (EA) on brain injury and expression of inflammatory factors in hippocampus after cardiopulmonary resuscitation in rats. Male Objective: SD rats were randomly divided into three groups: sham group, control group and EA group. Rats were asphyxiated for 8min before cardiopulmonary resuscitation (CPR). The EA group was inserted filiform needles in the shuigou and neiguan points and received electrical stimulation, while the control group was only inserted filiform needles at the same points. The survival rate of rat resuscitation and the recovery time of spontaneous circulation were recorded. Neurological deficit score (NDS) was measured at 24 h and 72 h after resuscitation. The spatial learning and memory ability of each group was tested by Morris water maze. Neuronal morphology and survival in hippocampus were observed by Nissl staining. The expression of inflammatory factors in hippocampus was detected by Western blot. Compared Results: with the sham group, the NDS of rats at 24 h and 72 h after resuscitation and the spatial learning and memory ability were significantly reduced in other two groups. In this two groups, hippocampal CA1 cells were disordered, meanwhile, the number of viable neurons, the expression of IL-10 were decreased, but IL-1 and IL-6 were increased when compared with sham group (P＜0.05). Compared with the control group, the survival rate was improved without statistically significant, the recovery time of spontaneous circulation was shortened (P＜0.05), the NDS was increased at 24 h and 72 h after resuscitation (P＜0.05), the escape latency of 6th day of water maze was shortened and the space exploration ability was promoted in the EA group (P＜0.05). The cell line in the hippocampal CA1 and the number of viable neurons in the EA group were significantly better than that of control group. IL-1 and IL-6 in the hippocampus of EA group were alleviated, while the expression of anti-inflammatory factor IL-10 was enhanced compared with the control group (P＜0.05).Conclusions: Electroacupuncture can alleviate brain injury after cardiopulmonary resuscitation in rats, and its protective effect may be related with inhibiting inflammatory factors and promoting the expression of anti-inflammatory factors. [ABSTRACT FROM AUTHOR]