渗透剂对白刺花体细胞胚成熟及萌发的影响.

To discuss the effect of different penetrants on maturation and germination of somatic embryo of Sophora davidii. Sucrose, maltose, sorbitol and PEG(6000)were used as penetrant in this paper. The development, maturation, and germination of somatic embryo was determined by different penetrant comparation. The results were as follows: the rate of somatic embryogenesis reached up to 66.21%, and the total number of embryos can achieve 79 when embryonic callus from hypocotyl of Sophora davidii were cultured in MS+ 2,4-D 0.2 mg·L-1 +NAA 1.0 mg·L-1+ 6-BA 2.0 mg·L-1 + TDZ 1.0 mg·L-1+sucrose 40 g·L-1+ glutamine 100 mg·L-1+plant gel 3 g·L-1. Adding 7% sucrose could promote the maturation rate of somatic embryo to 64.36%, meanwhile could also increase the development of polycotyledonous abnormal embryo. The maturation rate of somatic embryo went up as high as 88.89%, and the proportion of abnormal embryo the lowest, when somatic embryo were cultured in 2% maltose +2% sorbitol +4% sucrose combination medium. The maturation rate of somatic embryo was 82.35%, when the concentration of PEG was 30 g·L-1. Torpedo embryo was suitable for transported culture, the formative rate of somatic embryo was up to 90.58%. The rooting rate of mature somatic embryo was high, for 87.47% from three sugar combination culture. This study provides a theoretical reference and a feasible scheme for the development of somatic embryo seedling of Sophora davidii. [ABSTRACT FROM AUTHOR]