Hsa-miR-10a-5p 靶基因预测及生物信息学分析.

To provide theoretical basis for its experimental verification and regulation study, the target genes of miR-10a-5p were predicted and analyzed. The base sequence characteristics of miR-10a-5p of human, rat, mouse, and other animals were obtained and analyzed by miRBase database. The target genes of miR-10a-5p were predicted using the online databases Targetscan7.1, miRDB, mirDTP, and DIANA TOOLS, and the intersection of Wayne; maps was drawn using Venny 2.1. The GO function annotation and KEGG pathway analysis were performed on the intersection target genes using the online tool DAVID. The base sequence characteristics of miR-10a-5p of human, rat, mouse, and other species were obtained and analyzed by miRBase. Results show that the mature sequence of miR-10a-5p was highly conservative among different species. There were 79 target genes enriched in molecular function such as chromatin binding and transcription factor activity. These target genes were also involved in the biological development process of liver development and adipose tissue development, and they were mainly located in cellular signaling including eAMP pathway, TNF pathway, and AMPK pathway. In conclusion, the miR-10a-5p is involved in many parts of biological process and pathological process, especially the development process and carcinogenesis, which provides a biological basis for further research. [ABSTRACT FROM AUTHOR]