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Short-chain basement membrane collagen.
- Source :
-
European Journal of Biochemistry . 12/8/89, Vol. 186 Issue 1/2, p411-414. 4p. - Publication Year :
- 1989
-
Abstract
- The paper describes further characterization of the 55-kDa short-chain collagen from lens capsule. Lens capsules were extracted with 5.5 M guanidine · HCl and the extracted material was fractionated on agarose A5M followed by high-pressure liquid chromatography (HPLC). By amino acid composition, the major fraction obtained from HPLC was found to be different than type-IV collagen fragments. The 55-kDa short-chain collagen on pepsin digestion produced a 45-kDa pepsin-resistant fragment. The undifferentiated embryonal carcinoma (F-9) cells were found to synthesize increased amounts of 55-kDa short-chain collagen. The identity of this biosynthesized molecule with 55-kDa short-chain collagen from lens capsules was established by immunoprecipitation experiments. The results indicated a close similarity or identical nature of the short-chain collagens from these two sources. [ABSTRACT FROM AUTHOR]
- Subjects :
- *COLLAGEN
*GUANIDINES
*GUANIDINE
*AMINO acids
*PEPSIN
*CANCER cells
Subjects
Details
- Language :
- English
- ISSN :
- 00142956
- Volume :
- 186
- Issue :
- 1/2
- Database :
- Academic Search Index
- Journal :
- European Journal of Biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 13788066
- Full Text :
- https://doi.org/10.1111/j.1432-1033.1989.tb15224.x