持续压力负荷离体兔脊柱运动节段软骨终板的应力变化.

BACKGROUND: Changes of cartilage tissues have been shown to affect the function of intervertebral disc. OBJECTIVE: To observe the effect of continuous pressure load on the cartilage endplate of isolated rabbit spinal segment. METHODS: After the 16 New Zealand white rabbits were killed, the spinal movement segments were removed under the aseptic condition and randomly divided into control group (no pressure) and pressure group (continuous 29.4 N of pressure). The isolated culture was used in vitro and cultured in vitro. Before and after cultured for 3, 7 and 14 days, the 10 cartilage endplates in each group were removed for hematoxylin-eosin staining and expression of matrix metalloproteinase 13, collagen type II and proteoglycan were detected by immunohistochemistry, respectively. RESULTS AND CONCLUSION: (1) After 14 days of culture, the cartilage structure of the intervertebral disc endplate was almost intact, the number was reduced and tended to degenerate, which showed no significant changes compared with the control group. (2) The expression of matrix metalloproteinase 13 at 14 days after culture in the pressure group was increased compared with the baseline. The average absorbance value of matrix metalloproteinase 13 in the pressure group was significantly higher than that in the control group (P < 0.05). (3) The average absorbance value of collagen type II in the pressure group was significantly higher than that in the control group at 3 days after culture (P < 0.05). The expression of collage type II after 1 and 7 days of culture in the pressure group was significantly decreased compared with the baseline, which showed no significant difference from the control group. (4) The proteoglycan staining became slight in both groups at 3, 7 and 14 days after culture (P < 0.05). The average absorbance value of proteoglycan in the pressure group was significantly higher than that in the control group at 7 days (P < 0.05). (5) In summary, continuous pressure will lead to degeneration of endplate in vitro culture, which provides evidence for the early prevention and treatment of cartilage injury in intervertebral disc degeneration. [ABSTRACT FROM AUTHOR]