基于尿液代谢组学数据评价不同刮痧方案干预腰椎间盘突出症模型大鼠的效果

BACKGROUND: Scraping has been extensively applied in the treatment of lumbar disc herniation, and the underlying mechanism was explored by metabolomics. OBJECTIVE: To investigate the changes of endogenous metabolites in urine of rats with lumbar disc herniation and its mechanism of action by gas chromatography-mass spectrometry metabolomics method, and to analyze the difference in efficacy of different scraping schemes, in order to standardize the scraping therapy, and provide reference and basis for clinical application. METHODS: Forty-eight male Sprague-Dawley rats were randomly divided into blank, model and scraping groups (passing the sputum, acupuncture, sputum, and acupoint groups) (n=8 per group). The rat model of lumbar disc herniation was replicated by autologous nucleus pulposus transplantation. From the 5th day of modeling, the scraping group was intervened with different scraping schemes, once every other day, totally nine times. The study was approved by the Experimental Animal Ethics Committee of Nanjing University of Traditional Chinese Medicine in January 2017. RESULTS AND CONCLUSION: (1) Compared with before modeling, the mechanical pain threshold in the scraping and the model groups after modeling was decreased (P < 0.01). (2) A total of 15 potential biomarkers and 6 metabolic pathways were obtained after screening. After scraping, the content of 3-(3-hydroxyphenyl)propionic acid, aspartic acid and glutamic acid decreased, and the content of glycine, citric acid and cyclic adenosine increased, by adjusting alanine, aspartic acid and glutamine. The acid metabolism pathway was anti-inflammatory with analgesic, glycine, serine and threonine metabolism, glyoxylic acid and dicarboxylic acid metabolism, and a tricarboxylic acid cycle. (3) To conclude, the effect of regulating metabolites in the sputum group is better than that in the other three groups, which is presumed to be the best cure plan. [ABSTRACT FROM AUTHOR]