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Reproducibility of broth microdilution MICs for the novel siderophore cephalosporin, cefiderocol, determined using iron-depleted cation-adjusted Mueller-Hinton broth.
- Source :
-
Diagnostic Microbiology & Infectious Disease . Aug2019, Vol. 94 Issue 4, p321-325. 5p. - Publication Year :
- 2019
-
Abstract
- In 2017, the Clinical and Laboratory Standards Institute (CLSI) Subcommittee on Antimicrobial Susceptibility Testing approved the use of iron-depleted cation-adjusted Mueller-Hinton broth (ID-CAMHB) prepared with Chelex® 100 resin (Bio-Rad Laboratories, Hercules, CA) to determine MICs for cefiderocol. The current study examined the reproducibility of cefiderocol MICs generated for 19 clinical isolates of Gram-negative bacilli, with CAMHB produced by three manufacturers; each of the 19 isolates was tested for 10 replicates in ID-CAMHB from each manufacturer. When analyzed by individual media lot, greater than 95% of MIC results were within ± one doubling-dilution of the mode for each of the 19 isolates tested. The remaining 5.0% of MIC results were within ± two doubling-dilutions of the modal MIC. For all media lots combined, 92.2% of MIC results were within ± one doubling-dilution of the modal MIC for each isolate, 99.8% were within ± two doubling-dilutions and 100% were within three doubling-dilutions. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 07328893
- Volume :
- 94
- Issue :
- 4
- Database :
- Academic Search Index
- Journal :
- Diagnostic Microbiology & Infectious Disease
- Publication Type :
- Academic Journal
- Accession number :
- 137361063
- Full Text :
- https://doi.org/10.1016/j.diagmicrobio.2019.03.003