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Oroxylin A Suppresses the Cell Proliferation, Migration, and EMT via NF-κB Signaling Pathway in Human Breast Cancer Cells.
- Source :
-
BioMed Research International . 6/23/2019, p1-10. 10p. - Publication Year :
- 2019
-
Abstract
- Oroxylin A is a natural extract and has been reported to have a remarkable anticancer function. However, the mechanism of its anticancer activity remains not quite clear. In this study, we examined the inhibiting effects of Oroxylin A on breast cancer cell proliferation, migration, and epithelial-mesenchymal transition (EMT) and its possible molecular mechanism. The cytoactive and inflammatory factors were analyzed via Cell Counting Kit-8 assay and ELISA assay, respectively. Flow cytometry and western blotting were used to assess the cell proliferation. In addition, a wound healing assay and transwell assay were used to detect cell invasion and migration. qRT-PCR and western blot were employed to determine the effect of Oroxylin A on the EMT formation. Moreover, expression level of protein related to NF-κB signaling pathway was determined by western blot. The results revealed that Oroxylin A attenuated the cytoactivity of MDA-MB-231 cells in a dose- and a time-dependent manner. Moreover, cell proliferation, invasion, and migration of breast cancer cells were inhibited by Oroxylin A compared to the control. The mRNA and protein expression levels of E-cadherin were remarkably increased while N-cadherin and Vimentin remarkably decreased. Besides, Oroxylin A suppressed the expression of inflammatory factors and NF-κB activation. Furthermore, we also found that supplement of TNF-α reversed the effects of Oroxylin A on the cell proliferation, invasion, migration, and EMT in breast cancer cells. Taken together, our results suggested that Oroxylin A inhibited the cell proliferation, invasion, migration, and EMT through inactivating NF-κB signaling pathway in human breast cancer cells. These findings strongly suggest that Oroxylin A could be a therapeutic potential candidate for the treatment of breast cancer. [ABSTRACT FROM AUTHOR]
- Subjects :
- *CELL proliferation
*BIOLOGICAL assay
*CELL adhesion molecules
*CELL death
*CELL lines
*CELL physiology
*CELL motility
*CELLULAR signal transduction
*COLORIMETRY
*CYTOSKELETAL proteins
*ENZYME-linked immunosorbent assay
*EPITHELIAL cells
*FLOW cytometry
*GENE expression
*MEDICINAL plants
*MESSENGER RNA
*POLYMERASE chain reaction
*TUMOR necrosis factors
*WESTERN immunoblotting
*WOUND healing
*DNA-binding proteins
*PLANT extracts
*REVERSE transcriptase polymerase chain reaction
BREAST tumor prevention
Subjects
Details
- Language :
- English
- ISSN :
- 23146133
- Database :
- Academic Search Index
- Journal :
- BioMed Research International
- Publication Type :
- Academic Journal
- Accession number :
- 137123839
- Full Text :
- https://doi.org/10.1155/2019/9241769