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Structural determination and histochemical localization of ghrelin in the red-eared slider turtle, Trachemys scripta elegans
- Source :
-
General & Comparative Endocrinology . Aug2004, Vol. 138 Issue 1, p50-57. 8p. - Publication Year :
- 2004
-
Abstract
- We purified ghrelin peptide and determined the cDNA sequence encoding the precursor protein from the stomach of the red-eared slider turtle, Trachemys scripta elegans. The Trachemys ghrelin is comprised of 25-amino acids and has the sequence GSSFLSPEYQNTQQRKDPKKHTKLN. The third serine residue was modified by n-octanoic (C8:0), decanoic (C10:0) or unsaturated decanoic acid (C10:1). The carboxyl-terminal end of the peptide was not amidated, as seen in the ghrelins of other land vertebrates. Quantitative real-time PCR analysis revealed high levels of gene expression in the stomach and moderate levels in the large intestine and pancreas. Histochemical studies of turtle stomach revealed that ghrelin-immunopositive (ghrelin-ip) cells, which were small and round, were observed in the mucosal layer of the stomach but not in the myenteric plexus, and ghrelin-mRNA-expressing (ghrelin-ex) cells detected by in situ hybridization were scattered in a similar distribution as ghrelin-ip cells. These results indicate that ghrelin is present in reptiles. [Copyright &y& Elsevier]
Details
- Language :
- English
- ISSN :
- 00166480
- Volume :
- 138
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- General & Comparative Endocrinology
- Publication Type :
- Academic Journal
- Accession number :
- 13705222
- Full Text :
- https://doi.org/10.1016/j.ygcen.2004.05.005