Back to Search
Start Over
Generation of an infectious cDNA clone of an FMDV strain isolated from swine
- Source :
-
Virus Research . Sep2004, Vol. 104 Issue 2, p157-164. 8p. - Publication Year :
- 2004
-
Abstract
- A full-length cDNA clone of a foot-and-mouth disease virus (FMDV) isolated from swine was assembled in, the plasmid vector pBluescript II SK+ downstream of a T7 promoter. RNA synthesized in vitro using T7 polymerase lead to the production of infectious particles upon transfection of BHK-21 cells, as shown by cytopathic effects. The rescued virus was also found to be highly pathogenic for mice by intradermal injection producing a fatal disease indistinguishable from that of wild-type virus. The availability of this cDNA clone will allow examination of the molecular mechanisms behind FMDV virulence and attenuation, which might in turn allow the production of second-generation, genetically engineered FMDV vaccines. [Copyright &y& Elsevier]
- Subjects :
- *SWINE
*DNA
*NUCLEIC acids
*LIVESTOCK
Subjects
Details
- Language :
- English
- ISSN :
- 01681702
- Volume :
- 104
- Issue :
- 2
- Database :
- Academic Search Index
- Journal :
- Virus Research
- Publication Type :
- Academic Journal
- Accession number :
- 13704045
- Full Text :
- https://doi.org/10.1016/j.virusres.2004.04.002