Back to Search
Start Over
Prostaglandin E2 promotes nitric oxide synthase 2, platelet-activating factor receptor, and matrix metalloproteinase-2 expression in Escherichia coli-challenged ex vivo endometrial explants via the prostaglandin E2 receptor 4/protein kinase a signaling pathway
- Source :
-
Theriogenology . Aug2019, Vol. 134, p65-73. 9p. - Publication Year :
- 2019
-
Abstract
- Prostaglandin E 2 (PGE 2) is an inflammatory mediator involved in the pathogenesis of several chronic inflammatory conditions, including endometritis. Previous studies have shown that PGE 2 accumulates in Escherichia coli -challenged ex vivo endometrial explants, increasing the expression of pro-inflammatory factors and aggravating tissue damage; these alterations are linked to key enzymes involved in the synthesis of PGE 2 , including cyclooxygenases-2 (COX-2) and microsomal PGES-1 (mPGES-1). In this study, we aimed to investigate whether administration of PGE 2 modulated the activities of nitric oxide synthase 2 (NOS2), platelet-activating factor receptor (PAFR), and matrix metalloproteinase (MMP)-2 in E. coli- challenged ex vivo bovine endometrial explants. Our findings showed that COX-2 and mPGES-1 inhibitors significantly reduced NOS2, PAFR, and MMP-2 expression in the E. coli -challenged ex vivo endometrial explants. In addition, NOS2, PAFR, and MMP-2 expression levels were strongly increased in response to treatment with 15-prostaglandin dehydrogenase inhibitors in the E. coli- challenged ex vivo endometrial explants. However, these stimulatory effects could be blocked by PGE 2 receptor 4 (EP4) and protein kinase A (PKA) inhibitors. Overall, these findings show that pathogenic PGE 2 upregulated NOS2, PAFR, and MMP-2 expression, which may enhance inflammatory damage via the EP4/PKA signaling pathway in E. coli- challenged ex vivo endometrial explants. • Pathogenic bacteria (E. coli) infected bovine endometrial tissue in vitro. • PGE 2 promotes NOS2, PAFR, and MMP-2 expression in E. coli -infected bovine endometrial tissues in vitro. • PGE 2 promotes NOS2, PAFR, and MMP-2 expression via the prostaglandin E 2 receptor 4/protein kinase A signaling pathway. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 0093691X
- Volume :
- 134
- Database :
- Academic Search Index
- Journal :
- Theriogenology
- Publication Type :
- Academic Journal
- Accession number :
- 136984132
- Full Text :
- https://doi.org/10.1016/j.theriogenology.2019.04.028