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Multifunctional graphene supports for electron cryomicroscopy.
- Source :
-
Proceedings of the National Academy of Sciences of the United States of America . 6/11/2019, Vol. 116 Issue 24, p11718-11724. 7p. - Publication Year :
- 2019
-
Abstract
- With recent technological advances, the atomic resolution structure of any purified biomolecular complex can, in principle, be determined by single-particle electron cryomicroscopy (cryoEM). In practice, the primary barrier to structure determination is the preparation of a frozen specimen suitable for high-resolution imaging. To address this, we present a multifunctional specimen support for cryoEM, comprising large-crystal monolayer graphene suspended across the surface of an ultrastable gold specimen support. Using a low-energy plasma surface modification system, we tune the surface of this support to the specimen by patterning a range of covalent functionalizations across the graphene layer on a single grid. This support design reduces specimen movement during imaging, improves image quality, and allows highresolution structure determination with a minimum of material and data. [ABSTRACT FROM AUTHOR]
- Subjects :
- *ELECTRON cryomicroscopy
*ATOMIC structure
*GRAPHENE
Subjects
Details
- Language :
- English
- ISSN :
- 00278424
- Volume :
- 116
- Issue :
- 24
- Database :
- Academic Search Index
- Journal :
- Proceedings of the National Academy of Sciences of the United States of America
- Publication Type :
- Academic Journal
- Accession number :
- 136967349
- Full Text :
- https://doi.org/10.1073/pnas.1904766116