Study on lipids transfer in aqueous enzyme hydrolysis soybean protein and oil extraction process.

• The oil transfer was studied in the soybean emulsion hydrolyzed by protease. • The quantitative transfer of soybean oil extracted by petroleum ether was studied. • The organic extraction promoted the oil release and eliminated emulsification. • The process of enzyme-assisted aqueous extraction of soy oil was explored. • The process is lower economic cost and is easy to be industrialized. Enzyme-assisted aqueous Extraction of soybean oil is a promising green and safe alternative to traditional process. However, it is quite difficult to realize industrial application as the process needs large dosage of special enzyme and high-speed separation. To overcome these problems, the quantitative transfer of oil release during the hydrolysis of soybean protein by protease was studied. The results showed that the 1398 neutral protease with the total amount of only 720 IU/g soybean could be used to hydrolyze the soy protein, and the oil yield reached up to 90% after separation by the centrifugal force of 1960 × g. The enzymatic hydrolysis of soybean protein was beneficial to release and separate oil from soybean, and the separated protein could enrich almost 99.0% of oil from hydrolyzed emulsion. At the moment, the emulsification could be eliminated during the process of aqueous extraction with petroleum ether. [ABSTRACT FROM AUTHOR]