Back to Search
Start Over
Over-expression of Bgl1 from Aspergillus Niger in Penicillium Oxalicum.
- Source :
-
AIP Conference Proceedings . 2019, Vol. 2110 Issue 1, p020027-1-020027-6. 6p. - Publication Year :
- 2019
-
Abstract
- Penicillium oxalicum is the well-known major workhorse for cellulase production in industry. But the low β-glucosidase activity in P. oxalicum cellulase leads to inefficiency in biomass degradation and limits its further application, which is a major bottleneck in the efficient biomass conversion by cellulase. Previous study reported genetic transformations of P. oxalicum with homologous bgl genes. While, heterologously expressed beta-glucosidase by genetically modifying host strain could also be an effective tool in developing production of cellulases . In our study, the bgl1 gene of Aspergillus niger was heterologously overexpressed in Penicillium oxalicum. The resulted strain C3-1 showed 156.6- and 245.2-fold increased production of β-glucosidase relative to the parent strain PT3-1 and the wild-type (WT)strain 114-2, respectively. Besides, the filter paper assay of strain C3-1 increased at a factor of 8-folds compared to WT, resulting in levels of up to 3.2 U/ml. In summary, genetically engineering P. oxalicum signifcantly improves β-glucosidase activity, which will ultimately lead to a potent strategy to substantially boost the hydrolytic efficiency of the cellulase cocktail. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 0094243X
- Volume :
- 2110
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- AIP Conference Proceedings
- Publication Type :
- Conference
- Accession number :
- 136882450
- Full Text :
- https://doi.org/10.1063/1.5110821