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Huzhangoside A Suppresses Tumor Growth through Inhibition of Pyruvate Dehydrogenase Kinase Activity.
- Source :
-
Cancers . May2019, Vol. 11 Issue 5, p712. 1p. - Publication Year :
- 2019
-
Abstract
- Aerobic glycolysis is one of the important metabolic characteristics of many malignant tumors. Pyruvate dehydrogenase kinase (PDHK) plays a key role in aerobic glycolysis by phosphorylating the E1α subunit of pyruvate dehydrogenase (PDH). Hence, PDHK has been recognized as a molecular target for cancer treatment. Here, we report that huzhangoside A (Hu.A), a triterpenoid glycoside compound isolated from several plants of the Anemone genus, acts as a novel PDHK inhibitor. Hu.A was found to decrease the cell viability of human breast cancer MDA-MB-231, hepatocellular carcinoma Hep3B, colon cancer HT-29, DLD-1, and murine lewis lung carcinoma LLC cell lines. The activity of PDHK1 was decreased by Hu.A in both in vitro assays and in vivo assays in DLD-1 cells. Hu.A significantly increased the oxygen consumption and decreased the secretory lactate levels in DLD-1 cells. In addition, Hu.A interacted with the ATP-binding pocket of PDHK1 without affecting the interaction of PDHK1 and pyruvate dehydrogenase complex (PDC) subunits. Furthermore, Hu.A significantly induced mitochondrial reactive oxygen species (ROS) and depolarized the mitochondrial membrane potential in DLD-1 cells. Consistently, when Hu.A was intraperitoneally injected into LLC allograft mice, the tumor growth was significantly decreased. In conclusion, Hu.A suppressed the growth of tumors in both in vitro and in vivo models via inhibition of PDHK activity. [ABSTRACT FROM AUTHOR]
- Subjects :
- *REACTIVE oxygen species
*ANIMAL experimentation
*BIOLOGICAL transport
*BREAST tumors
*CELL lines
*COLON tumors
*GLYCOSIDES
*HEPATOCELLULAR carcinoma
*INTRAPERITONEAL injections
*LACTATES
*LUNG tumors
*MICE
*MITOCHONDRIA
*MOLECULAR structure
*PROTEIN kinases
*TERPENES
*OXYGEN consumption
*CELL survival
*IN vitro studies
*IN vivo studies
Subjects
Details
- Language :
- English
- ISSN :
- 20726694
- Volume :
- 11
- Issue :
- 5
- Database :
- Academic Search Index
- Journal :
- Cancers
- Publication Type :
- Academic Journal
- Accession number :
- 136751067
- Full Text :
- https://doi.org/10.3390/cancers11050712