Non-enzymatic sensing of dopamine by localized surface plasmon resonance using carbon dots-functionalized gold nanoparticles.

• A new colorimetric sensor based on CDs-Au NPs was developed. • Aggregation of the S-CD functionalized Au NPs in the presence of DA and Fe3+. • The decrease in LSPR band of S-CD@Au NPs at 520 nm and appearance of a band at 670 nm. • The ratio of the LSPR band at 670 nm to 520 nm was proportional to DA concentration. • The proposed sensor was applied for determination of DA in biological samples. A highly selective, sensitive, and rapid colorimetric sensor for the determination of dopamine (DA) was developed using the color change of S-doped carbon dots functionalized gold nanoparticles (S-CDs@Au NPs). The base of the method is the formation of a complex between the amine groups of dopamine with carboxylic groups of S-CDs@Au NPs followed by their aggregation with Fe3+ ions which acts as the linkers causing a red shift from 520 to 670 nm in the localized surface plasmon peak of S-CDs@Au NPs. The ratio of absorbance intensity at 670 nm to 520 nm was monitored as the analytical signal for determination of dopamine. The parameters affecting the analytical signal including reaction time, solution pH, the concentration of Au NPs and concentration of Fe3+ were optimized. At optimized conditions, the calibration curve was linear in the concentration range of 0.81–16.80 μM of dopamine. The detection and quantification limits were 0.23 μM and 0.77 μM, respectively. The intra-day and inter-day relative standard deviation (RSDs) at 5.0 μM of DA were 3.9% and 5.6%, respectively (n = 5). The applicability of the method for determination of DA in dopamine ampoule, urine and serum human samples was investigated. [ABSTRACT FROM AUTHOR]