Raman spectroscopic discrimination of normal and cancerous lung tissues.

Raman spectroscopy is non-destructive method that allows monitoring of biological tissues with minimal intervention. FT-Raman (λ ex 1064 nm) and NIR-Vis-Raman (λ ex 785 nm) spectroscopic measurements were used in ex vivo analysis of normal, non-cancerous abnormal and cancerous lung tissues. Spectroscopic discrimination of the lung tissue samples was made by the use of the ratio of characteristic bands and multivariate statistical methods (PCA, LDA). The combination of Raman spectroscopy and multivariate statistics may have a diagnostic potential for recognizing of cancer lesions in lung. Unlabelled Image • Raman spectroscopy allows monitoring of biological tissues with minimal intervention. • Raman measurements at λ ex 1064 nm and 785 nm were used in ex vivo analysis of lung tissues. • Normal, non-cancerous abnormal and cancerous samples were discriminated by statistical evaluation of the spectra. • Raman spectroscopy may have a diagnostic potential for recognizing of cancer lesions in lung. [ABSTRACT FROM AUTHOR]