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MiR-125b regulates the proliferation and metastasis of triple negative breast cancer cells via the Wnt/β-catenin pathway and EMT.
- Source :
-
Bioscience, Biotechnology & Biochemistry . Jun2019, Vol. 83 Issue 6, p1062-1071. 10p. - Publication Year :
- 2019
-
Abstract
- Background/aim: MiR-125b plays an important role in breast cancer. The current study was to explore the expression and function of miR-125b in triple negative breast cancer cells. Materials and methods: The expression of miR-125b in human TNBC samples and cell lines were examined by qRT-PCR. MTT, scratch assays and transwell assays were utilized to observe the proliferation, migration and invasion ability. MiR-125b's target gene and downstream signaling pathways were investigated by Luciferase Reporter Assays, qRT-PCR, immunofluorescence assays and western bolt. Results: MiR-125b was highly expressed in human TNBC tissues and cell lines. Inhibiting miR-125b expression suppressed the proliferation, cell migration and invasion. The three-prime untranslated region (3´-UTR) of adenomatous polyposis coli (APC) mRNA contains miR-125b binding sites, and inhibiting miR-125b expression suppressed the activity of the intracellular Wnt/β-catenin pathways and EMT. Conclusion: Inhibiting miR-125b regulates the Wnt/β-catenin pathway and EMT to suppress the proliferation and migration of MDA-MB-468 TNBC cells. MiR-125b was highly expressed in human TNBC tissues and cell lines. Inhibiting miR-125b expression suppressed the proliferation, cell migration and invasion of MDA-MB-468 TNBC cells by targeting adenomatosis polyposis coli (APC) to regulate the Wnt/β-catenin pathway and EMT. [ABSTRACT FROM AUTHOR]
- Subjects :
- *CELL proliferation
*METASTASIS
*CATENINS
Subjects
Details
- Language :
- English
- ISSN :
- 09168451
- Volume :
- 83
- Issue :
- 6
- Database :
- Academic Search Index
- Journal :
- Bioscience, Biotechnology & Biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 136380108
- Full Text :
- https://doi.org/10.1080/09168451.2019.1584521