黄芪红花配伍对大鼠脑缺血后血管新生 及Cav-1/VEGF信号通路的影响.

Objective: To explore whether Huangqi-Honghua combination protect brain from cerebral ischemia reperfusion injury through promoting angiogenesis by inducing the Cav-1/VEGF(Caveolin-1/vascular endothelial growth factor) signal pathway. Methods:Sixty rats were randomly divided into five groups: control group, model group and Huangqi-Honghua 40:1, 20:1, 5:1 groups(12 rats per group). The rat cerebral ischemia reperfusion injury models were induced by nylon monofilament. After 21 days of administration, the neurological scores were evaluated, the infarct volumes were calculated. Immunohistochemical method was used to detect the microvessel density(MVD) in brain tissue. RT-PCR and Western-blotting was used to detect the m RNA and protein expression levels of Cav-1 and VEGF. Results: After 21 days of administration, the neurological scores of each group were decreased, especially the Huangqi-Honghua groups(P<0.01), and infarct volume(P<0.05~ P<0.01)in Huangqi-Honghua groups were significantly lower than the model group. Compared with model group, the level of MVD, Cav-1 and VEGF m RNA and protein expression levels were significantly higher in Huangqi-Honghua treatment group(P<0.05~ P<0.01). Conclusions: Huangqi-Honghua combination has protective against cerebral ischemia injury, the Cav-1/VEGF pathway may be involved and then promote the angiogenesis. Additionally, the best proportion of Huangqi-Honghua was 5:1. [ABSTRACT FROM AUTHOR]