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Molybdenum disulfide-integrated photonic barcodes for tumor markers screening.

Authors :
Bian, Feika
Sun, Lingyu
Cai, Lijun
Wang, Yu
Zhao, Yuanjin
Wang, Shuqi
Zhou, Mengtao
Source :
Biosensors & Bioelectronics. 5/15/2019, Vol. 133, p199-204. 6p.
Publication Year :
2019

Abstract

As a new class of two-dimensional (2D) materials, molybdenum disulfide (MoS 2) has huge potential in biomedical area; while its applications in multiplex bioassays are still a challenge. Here, we present novel MoS 2 -integrated silica colloidal crystal barcode (SCCB) for multiplex microRNA (miRNA) screening. MoS 2 was adsorbed on SCCBs by electrostatic interaction, and quantum dots (QDs) decorated hairpin probes were coupled on MoS 2 by covalent linkage. As the MoS 2 could quench the QDs of the hairpin probes, they together formed a molecular beacon (MB) structure before the detection. When used in assays, target miRNA could form a double strand with the probe and made QDs keep away from MoS 2 sheets to recovery their fluorescence. Because the released QDs were positively correlated with the concentration of the hybridized nucleic acid, the target miRNAs could be quantified by measuring the fluorescence signal of the QDs on the SCCBs. In addition, by utilizing different MoS 2 -integrated structural color encoded SCCBs, multiplexed miRNA quantification could also be realized. Based on this strategy, we have demonstrated that several pancreatic cancer-related miRNAs could be selectivity and sensitivity detected with a detection limit of 4.2 ± 0.3 nM. These features make the MoS 2 -integrated SCCB ideal for many potential applications. • A novel MoS 2 -integrated silica colloidal crystal barcodes (SCCBs) for multiplex miRNA screening. • MoS 2 sheets on SCCBs and the QD-decorated hairpin probes formed a molecular beacon (MB) structure for target detection. • MiRNAs could be quantified by measuring the fluorescent signal which positively correlated with their concentration. • The label-free strategy demonstrated good accuracy and high sensitivity multiplexed miRNA quantification. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
09565663
Volume :
133
Database :
Academic Search Index
Journal :
Biosensors & Bioelectronics
Publication Type :
Academic Journal
Accession number :
136178796
Full Text :
https://doi.org/10.1016/j.bios.2019.02.066