不同冻存条件对甘蔗原生质体活力和再生能力的影响.

In order to obtain high vitality and regeneration ability of sugarcane protoplast, this experiment was done to study the protoplast of frozen storage liquid combination, frozen storage temperature, frozen storage time and recovery temperature. The results were as follows; (1) Different eryopreservations, different eryopreservation temperatures and different protoplast sources of different materials had significant differences on the vitality of the protoplast of sugarcane. Compared with the combination of three frozen liquid deposits, in Combination 2 (70% medium +20% serum + 10% DMSO) , the recovery activity was the strongest alter 30 <1, as high as 72%. Cryopreserved recovery within 90 d, liquid nitrogen -80 °C and -196 Ai freezer, sugarcane protoplast energy difference was not significant, and the vigor was above 75%. But after 90 d of frozen-storage, the protoplast dynamic at -196 °C was stronger than -80 °C after recovery. For different materials, the protoplast dynamic of the young leaves frozen stored for 30 d was up to 79.2%, and the protoplast dynamic of stem tip frozen for 30 d was only 42.7%. (2) There was no significant difference in the time between the first initiation of division and the formation of cell mass in the treatments with different eryopreservation liquids and different eryopreservation temperatures. After the protoplasts were cultured for about 5-6 d, the cell wall was basically complete, and the cells began to divide after 6 d, and the cell masses formed after 15 d of culture. For different materials, the strongest regeneration ability was found in the protoplasts from stem tip by enzymatic hydrolysis, which cell wall formation showed 3 d earlier, and 2 d earlier in the first cell division than those from the juvenile leaves. [ABSTRACT FROM AUTHOR]