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Effects of CKMT1 on radiosensitivity of nasopharyngeal carcinoma cells.
- Source :
-
International Journal of Radiation Biology . May2019, Vol. 95 Issue 5, p597-606. 10p. - Publication Year :
- 2019
-
Abstract
- Purpose: Radioresistance is an important factor for unsatisfactory prognosis in Nasopharyngeal carcinoma (NPC) patients. Ubiquitous mitochondrial creatine kinase (CKMT1) is always associated with malignancy in a variety of cancers. However, its significance in NPC progression and radiosensitivity remains unclear. The present study focused on investigating the effects of CKMT1 on NPC cell radiosensitivity. Material and methods: CKMT1 was overexpressed in NPC cell line CNE-1 or knocked out in CNE-2. Biological changes were detected after cells exposing to different doses of X-ray to determine the role of CKMT1 on NPC cell radiosensitivity. Results: CKMT1 promotes proliferation and migration in NPC cell lines CNE-1 and CNE-2. Overexpression of CKMT1 in CNE-1 cells enhanced colony formation rates, reduced G2/M phase cell cycle arrest, lowered apoptosis rate and c-PARP level, and elevated STAT3 phosphorylation level after radiation treatment. While knocking out CKMT1 using the CRISPR/Cas9 system in CNE-2 cells lowered colony formation rates, increased G2/M phase cell cycle arrest, apoptosis rates, and c-PARP levels, and decreased STAT3 phosphorylation in response to radiation treatment. Conclusions: NPC cells with higher CKMT1 exhibited lower radiosensitivity through promoting phosphorylation of STAT3. Our findings suggest that CKMT1 may be an alternative radiotherapeutic target in NPC therapy. [ABSTRACT FROM AUTHOR]
- Subjects :
- *CREATINE kinase
*CELL migration
*CELL cycle
*CELLS
*CARCINOMA
Subjects
Details
- Language :
- English
- ISSN :
- 09553002
- Volume :
- 95
- Issue :
- 5
- Database :
- Academic Search Index
- Journal :
- International Journal of Radiation Biology
- Publication Type :
- Academic Journal
- Accession number :
- 136150136
- Full Text :
- https://doi.org/10.1080/09553002.2019.1554919