有氧耐力训练高脂诱导肥胖模型小鼠白色脂肪组织和血浆PPARγ的水平变化.

BACKGROUND: Peroxisome proliferators activated receptor γ has been shown to be a key gene regulating adipocyte differentiation. Endurance exercise training is a very effective means of weight loss, but the effect of endurance exercise on the plasma peroxisome proliferators activated receptor γ content and the expression of peroxisome proliferators activated receptor γ protein in adipose tissue of nutritional obese mice remains to be determined. OBJECTIVE: To explore the changes of peroxisome proliferators activated receptor γ protein expression in white adipose tissue and plasma peroxisome proliferators activated receptor γ content in high fat-induced obese mice after moderate-intensity endurance exercise training. METHODS: Forty male C57BL/6J mice of 4 weeks old were provided by Beijing Weitong Lihua Experimental Animal Technology Co., Ltd. and were randomly divided into control group (n=10) and high fat induction group (n=30) after 1 week of adaptive feeding. After high-fat-induced mice were fed with high-fat diet for 8 weeks, 20 mice with larger body weight were divided into obese model group (n=10) and endurance exercise group (n=10) according to S-type grouping. Control group mice were fed with normal diet, no exercise. Mice in the obese model and endurance exercise groups were fed with high fat diet. Mice in the obese model group did not exercise. Mice in the endurance exercise group were subjected to 1 week of adaptive exercise, and then for 8 weeks of moderate-intensity endurance exercise training, 5 times weekly, 60 min/time. At the end of the experiment, the body weight of the mice was weighed, and the plasma level of peroxisome proliferators activated receptor γ was detected. The peripheral and epididymal adipose tissue was obtained and weighed. Part of the adipose tissue was taken for hematoxylin-eosin staining, and the remaining adipose tissue was detected by western blotting for peroxisome proliferators activated receptor γ protein expression. RESULTS AND CONCLUSION: (1) High fat induction could significantly increase the body weight of mice, causing the increase of adipose tissue weight and fat cells in the body. (2) High fat induction could significantly reduce plasma peroxisome proliferators activated receptor γ content and adipose tissue peroxisome proliferators activated receptor γ protein expression. (3) Moderate-intensity endurance exercise training could significantly reduce the body weight of obese mice induced by high fat, significantly reduce the weight of adipose tissue, and significantly reduce the volume of fat cells. (4) Moderate-intensity endurance exercise training can significantly increase the content of peroxisome proliferators activated receptor γ in plasma and significantly increase the expression of peroxisome proliferators activated receptor γ protein in adipose tissue. It is indicated that moderate-intensity endurance exercise training may achieve the purpose of reducing body weight by increasing the content of peroxisome proliferators activated receptor γ in mouse plasma and the expression level of peroxisome proliferators activated receptor γ protein in adipose tissue. [ABSTRACT FROM AUTHOR]