Back to Search
Start Over
Studies on Soybean Trypsin Inhibitors 3. Amino-Acid Sequence of the Carboxyl-Terminal Region and the Complete Amino-Acid Sequence of Soybean Trypsin Inhibitor (Kunitz).
- Source :
-
European Journal of Biochemistry . 1973, Vol. 32 Issue 3, p417-431. 15p. - Publication Year :
- 1973
-
Abstract
- For the elucidation of the amino-acid sequence of the carboxyl-terminal region of soybean trypsin inhibitor (Kunitz), fragments C and D were digested with trypsin, and the resulting peptides were separated by ion-exchange chromatography on Dowex 50X2 or by gel filtration on Bio-Gel P-4. Further fractionation and purification of the peptides were performed by ion-exchange chromatography on Dowex 1X2, by gel filtration on Bio-Gel P-2 or by high-voltage paper electrophoresis at pH 1.9 and 3.6. Three peptides were obtained in pure form from fragment C and ten peptides from fragment D, and their amino-acid sequences were determined by the direct Edman method and by carboxy- peptidase digestion technique. Overlapping peptides necessary for the alignment of the tryptic peptides from fragment D were obtained from a chymotryptic hydrolysate of fragment D. Nine main peptides and rune minor peptides were obtained. The amino acid composition and the partial amino-acid sequence of the 18 chymotryptic peptides of fragment D made it possible to establish the amino-acid sequence of the carboxyl-terminal region of the inhibitor. The complete amino-acid sequence of soybean trypsin inhibitor (Kunitz) deduced here was compared with that of Bowman-Birk inhibitor, another well-known soybean proteinase inhibitor. [ABSTRACT FROM AUTHOR]
- Subjects :
- *SOYBEAN
*TRYPSIN
*AMINO acids
*PEPTIDES
*CHROMATOGRAPHIC analysis
*PROTEINS
Subjects
Details
- Language :
- English
- ISSN :
- 00142956
- Volume :
- 32
- Issue :
- 3
- Database :
- Academic Search Index
- Journal :
- European Journal of Biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 13596202
- Full Text :
- https://doi.org/10.1111/j.1432-1033.1973.tb02624.x