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Studies on Soybean Trypsin Inhibitors 3. Amino-Acid Sequence of the Carboxyl-Terminal Region and the Complete Amino-Acid Sequence of Soybean Trypsin Inhibitor (Kunitz).

Authors :
Koide, Takehiko
Ikenaka, Tokuji
Source :
European Journal of Biochemistry. 1973, Vol. 32 Issue 3, p417-431. 15p.
Publication Year :
1973

Abstract

For the elucidation of the amino-acid sequence of the carboxyl-terminal region of soybean trypsin inhibitor (Kunitz), fragments C and D were digested with trypsin, and the resulting peptides were separated by ion-exchange chromatography on Dowex 50X2 or by gel filtration on Bio-Gel P-4. Further fractionation and purification of the peptides were performed by ion-exchange chromatography on Dowex 1X2, by gel filtration on Bio-Gel P-2 or by high-voltage paper electrophoresis at pH 1.9 and 3.6. Three peptides were obtained in pure form from fragment C and ten peptides from fragment D, and their amino-acid sequences were determined by the direct Edman method and by carboxy- peptidase digestion technique. Overlapping peptides necessary for the alignment of the tryptic peptides from fragment D were obtained from a chymotryptic hydrolysate of fragment D. Nine main peptides and rune minor peptides were obtained. The amino acid composition and the partial amino-acid sequence of the 18 chymotryptic peptides of fragment D made it possible to establish the amino-acid sequence of the carboxyl-terminal region of the inhibitor. The complete amino-acid sequence of soybean trypsin inhibitor (Kunitz) deduced here was compared with that of Bowman-Birk inhibitor, another well-known soybean proteinase inhibitor. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
00142956
Volume :
32
Issue :
3
Database :
Academic Search Index
Journal :
European Journal of Biochemistry
Publication Type :
Academic Journal
Accession number :
13596202
Full Text :
https://doi.org/10.1111/j.1432-1033.1973.tb02624.x