产阿扎霉素F菌株Streptomyces malaysiensis种子培养条件研究.

【Objective】 In order to increase the biomass of the strain S. malaysiensis ECO-00002, the seed medium composition and culture conditions were optimized. 【Method】 The Plackett-Burman test was used to screen the carbon and nitrogen sources for seed culture medium, and the Response Surface Analysis test was used to identify the most significant factors and to determine their optimal values. Furthermore, the proportion of multivitamins, complex salts, and CaCO3 were determined. The initial pH of the medium and liquid volume were optimized by comparative tests. 【Result】 The optimization results showed that the growth of the strain seed mainly affected by the carbon source which were glucose, yeast extract and malt extract, and the maximum response values of the three factors were 1.45 %, 1.50 %, 2.00 %, respectively. The inorganic salt was as follows: CaCO3 3.00 g/L, MgSO4·7 H20 1.00 g/L, MnSO4·4 H20 0.10 g/L, KH2PO4 0.20 g/L. Initial pH was 7.50, and liquid volume was 100 mL/500 mL. The optimization of culture conditions was as following: culture temperature was 28 °C, and incubation time was 200 r/min with shaking culture 40 hours. 【Conclusion】 Under the optimized seed culture conditions, the biomass yield increased from 4.09 g to 8.91 g, and the increase rate was 117.70 %. The optimization effect was significant. [ABSTRACT FROM AUTHOR]