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Establishment of a Transgenic Tobacco Cell Suspension Culture System for Producing Murine Granulocyte-Macrophage Colony Stimulating Factor.
- Source :
-
Molecules & Cells (Elsevier B.V) . 1997, Vol. 7 Issue 6, p783-787. 5p. 1 Black and White Photograph, 1 Diagram, 2 Graphs. - Publication Year :
- 1997
-
Abstract
- We tested if murine granulocyte-macrophage colony stimulating factor (mGM-CSF) is produced as a biologically active form through plant cell culture. The mGM-CSF gene was cloned into the plant expression vector, pBI121, and Ti-plasmid mediated transformation of tobacco leaves was conducted using Agrobacterium tumefaciens harboring the recombinant mGM-CSF (rmGM-CSF) gene. Cell suspension culture was established from the leaf-derived calli of transgenic tobacco plants. Northern blot analysis indicated the expression of the introduced mGM-CSF gene in both transgenic plant and cell suspension cultures. In addition, the biological activity of rmGM-CSF from plant cell culture was confirmed by measuring the proliferation of GM-CSF dependent FDC-Pl cells. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 10168478
- Volume :
- 7
- Issue :
- 6
- Database :
- Academic Search Index
- Journal :
- Molecules & Cells (Elsevier B.V)
- Publication Type :
- Academic Journal
- Accession number :
- 135879502
- Full Text :
- https://doi.org/10.1016/s1016-8478(23)13379-6