اثر تمرین ورزشی و محدودیت کالری بر بیان ژن سوبسترای گیرنده انسولین- 1 در آدیپوسیتهای موشنر چاق

Introduction: Sedentary life style and high calorie foods lead to obesity, hence the aim of the study was to investigate the effects of different doses of exercise and caloric restriction on insulin receptor substrate-1 in obese male rats. Methods and Materials: 48, eight-week-old male Wistar rats (190 ± 16 g) fed high-fat palate for seventeen weeks. After obesity induction, the obesity group was randomly assigned to one of the six groups: EXE, CR, EXCR1, EXCR2, and EXCR3 as experimental groups and OC as the control group. Then 10% of food intake was reduced. Exercise intensity was adjusted about 70-75% vo2max, 28 m/min for 48 to 55 minute. Negative energy balance was applied through caloric restriction (CR) (6d/w), exercise training (EXE) (6d/w), and in three combinations: EXCR1 (2d/w exercise + 4d/w CR,) EXCR2 (3d/w exercise + 3d/w CR) and EXCR3 (4d/w exercise + 2d/w CR) for two month. One way ANOVA and Tukey post-hoc test was applied for data analysis by SPSS version 16 (p<0.05). Results: IRS-1 mRNA expression was significantly higher in CR in comparison to OC (p=0.024). Also, IRS-1 expression significantly increased in EXCR2 comparing to OC (p=0.033). Conclusion: 10% of negative energy balance in forms of caloric restriction per se or in conjunction with exercise can provoke mRNA expression of insulin substrate receptor-1 that may lead to insulin sensitivity improvement. [ABSTRACT FROM AUTHOR]