Biomolecule Sensor Based on Azlactone‐Modified Hydrogel Films.

A 3D hydrogel layer is probed by combining surface plasmon resonance with optical waveguide spectroscopy to detect biomolecules. A template terpolymer P(DMAAm‐co‐DMIAAm‐co‐VDMA) is synthesized via reversible addition‐fragmentation chain‐transfer polymerization. The terpolymer is then modified with an amino group bearing biotin to enable biomolecular recognition for streptavidin. A hydrogel thin layer is prepared onto a gold surface after spin‐coating and photo‐crosslinking of the modified polymer. Finally, the hydrogel is utilized to quantitatively detect streptavidin by using surface plasmon resonance–optical waveguide spectroscopy measurements. An azlactone‐supported terpolymer is synthesized via reversible addition‐fragmentation chain‐transfer polymerization and then modified with a primary amine group bearing biotin to obtain a functional terpolymer. A hydrogel film constructed by the terpolymer is able to form a sensor chip to quantitatively detect streptavidin by using surface plasmon resonance–optical waveguide spectroscopy. [ABSTRACT FROM AUTHOR]