通过构建竞争性内源 RNA 网络识别潜在的椎间盘疾病相关 circRNA.

CircRNAs can reduce the inhibitory effect of miRNA on other target RNAs and indirectly regulate its expression level by competitive binding of small RNAs to miRNAs. This competitive relationship represents a novel gene regulation mechanism that plays an important role in cancer physiology and development. Using the bioinformatics method, we reannotated the gene expression profile and circRNA probe profile. With the miRNA target information predicted by the MiRanda algorithm,we constructed a competitive endogenous RNAs network and found five important modules related to the disease. The relations of the two ceRNAs, i.e. CD74 and hsacirc0001320 mediated by hsa-miR-17-3p and PAPSS2 and hsa_circ_0000077 mediated by hsa-let-7a-2-3p, play an important role in regulating the degeneration of intervertebral disc and thus become a potential clinical marker. Further, we predicted the biological functions of the two circRNAs by annotating the function of the target gene, which was significantly related to the inflammatory reaction of the intervertebral disc and bone development. It provides basis for clinical gene detection to predict disease and drug target therapy, as well as ideas for scientific research of intervertebral disc disease. [ABSTRACT FROM AUTHOR]