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Production of prostaglandin F2α by molecular breeding of an oleaginous fungus Mortierella alpina.
- Source :
-
Bioscience, Biotechnology & Biochemistry . Apr2019, Vol. 83 Issue 4, p774-780. 7p. - Publication Year :
- 2019
-
Abstract
- Cyclooxygenases are responsible for the production of prostaglandin H2 (PGH2) from arachidonic acid. PGH2 can be converted into some bioactive prostaglandins, including prostaglandin F2α (PGF2α), a potent chemical messenger used as a biological regulator in the fields of obstetrics and gynecology. The chemical messenger PGF2α has been industrially produced by chemical synthesis. To develop a biotechnological process, in which PGF2α can be produced by a microorganism, we transformed an oleaginous fungus, Mortierella alpina 1S-4, rich in triacylglycerol consisting of arachidonic acid using a cyclooxygenase gene from a red alga, Gracilaria vermiculophylla. PGF2α was accumulated not only in the mycelia of the transformants but also in the extracellular medium. After 12 days of cultivation approximately 860 ng/g and 6421 µg/L of PGF2α were accumulated in mycelia and the extracellular medium, respectively. The results could facilitate the development of novel fermentative methods for the production of prostanoids using an oleaginous fungus. [ABSTRACT FROM AUTHOR]
- Subjects :
- *PROSTAGLANDINS
*MORTIERELLA
*CYCLOOXYGENASES
Subjects
Details
- Language :
- English
- ISSN :
- 09168451
- Volume :
- 83
- Issue :
- 4
- Database :
- Academic Search Index
- Journal :
- Bioscience, Biotechnology & Biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 135477071
- Full Text :
- https://doi.org/10.1080/09168451.2018.1562880