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Evaluation of Different Stabilizers and Inactivating Compounds for the Enhancement of Vero Cell Rabies Vaccine Stability and Immunogenicity: In Vitro Study.

Authors :
Olayan, Ebtesam
El-Khadragy, Manal
Mohamed, Aly Fahmy
Mohamed, Amany Khalil
Shebl, Rania Ibrahim
Yehia, Hany M.
Source :
BioMed Research International. 3/17/2019, p1-9. 9p.
Publication Year :
2019

Abstract

Inactivation of rabies virus is essential for rabies vaccine preparation where the inactivating compound that is currently recommended for rabies vaccine preparation is β-propiolactone (β-PL). This compound is considered better than phenol and formalin but it is expensive and potentially carcinogenic. Data revealed that Ascorbic acid (AA) with cupric ions could yield complete and irreversible inactivation of rabies virus without adversely affecting its antigenicity. Additionally, the results of testing the vaccine potency with the selected inactivating compounds were comparable (P<0.05), and ED50 was higher than the recommended World Health Organization (WHO) limits. The use of HemaGel (plasma substitute) for testing vaccine stabilization was compared with the currently used vaccine stabilizers (human albumin and lactose). HemaGel yielded better stability than the other tested stabilizers. Monitoring of cellular and humoral immune responses indicated that both the total IgG level against rabies vaccine and the IFN and IL5 levels obtained with the HemaGel-stabilized vaccines were higher than those obtained with human albumin- and lactose-stabilized vaccine candidates. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
23146133
Database :
Academic Search Index
Journal :
BioMed Research International
Publication Type :
Academic Journal
Accession number :
135370527
Full Text :
https://doi.org/10.1155/2019/4518163