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Ultra-low aspect ratio spiral microchannel with ordered micro-bars for flow-rate insensitive blood plasma extraction.
- Source :
-
Sensors & Actuators B: Chemical . May2019, Vol. 287, p320-328. 9p. - Publication Year :
- 2019
-
Abstract
- Highlights • An ultra-low aspect ratio spiral system used for flow-rate insensitive particle/cell focusing was developed. • Various-degree enhancements of secondary flows were established by geometric confinement. • The single-layer system allows easy-to-operation and highly-efficient plasma extraction under a broad flow capacity. • A high throughput and rejection efficiency of blood cells can be achieved in a manual operation. Abstract The ability to regulate secondary flow is significant for efficient particle/cell focusing. Microfluidic technologies have accomplished impressive progress in particle/cell manipulation with the help of the Dean effect. Herein, we explored blood cell focusing with the ultra-low aspect ratio inertial microfluidic system which uses the geometric confinement to enhance the secondary flows to different degrees. Introducing a series of micro-bars in the spiral microchannels accelerates the secondary flow, which can greatly enhance highly-efficient particle/cell focusing under various flow rates. Further, plasma extraction can be successfully achieved from 15× diluted whole blood in an easy-to-use (without the assistance of sheath fluid and complex manufacturing of multi-layer structure), high and wide flow rates (1–5 mL/min, exhibiting no parallel construction design), long-term (at least 60 min), stable (processing at least 300 mL blood samples with consistent efficiency), and highly-efficient (99.99% blood cell rejection ratio) manner. Compared with previously-reported technologies, the engineering strategy of secondary flow in our designed dimension-confined spiral channel provides a balanced overall performance for plasma separation pointing to ease-to-fabrication, insensitive to flow-rate, high throughput and operation efficiency. [ABSTRACT FROM AUTHOR]
- Subjects :
- *BLOOD plasma
*BLOOD cells
*FINANCIAL ratios
Subjects
Details
- Language :
- English
- ISSN :
- 09254005
- Volume :
- 287
- Database :
- Academic Search Index
- Journal :
- Sensors & Actuators B: Chemical
- Publication Type :
- Academic Journal
- Accession number :
- 135351344
- Full Text :
- https://doi.org/10.1016/j.snb.2019.02.066