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Characterization of novel exopolysaccharide of Enterococcus faecium WEFA23 from infant and demonstration of its in vitro biological properties.
- Source :
-
International Journal of Biological Macromolecules . May2019, Vol. 128, p710-717. 8p. - Publication Year :
- 2019
-
Abstract
- Abstract In this study exopolysaccharide (EPS) of Enterococcus faecium WEFA23 from healthy infant's feces was yielded as high as 130 mg/L by fermentation. By purification the EPS was further fractioned into A23-1, A23-2, A23-3 and A23-4 on HiTrap Q HP and Superdex G-200 column. As the major purified fractions, A23-2 and A23-4 were analyzed for the preliminary structures and investigated for the biological properties in vitro. The molecular weight of A23-2 and A23-4 was 2.50 × 104 Da and 3.23 × 104 Da, respectively. A23-2 was composed of mannose, glucose and galactose with a ratio of 1.38:1.00:1.42, while A23-4 consisted of only mannose. The antioxidant ability of A23-2 was significantly higher than that of A23-4, as proved by scavenging test of DPPH radical, hydroxyl radical and superoxide radical. Both A23-2 and A23-4 exhibited strong inhibition against the adhesion of L. monocytogenes CMCC54007 on HT-29 cells no matter for the styles of competition, displacement and exclusion. Notably, for exclusion they had a maximal inhibition rate of 89.85 ± 0.46% and 90.81 ± 2.29%, respectively, at 200 μg/mL. Highlights • Novel exopolysaccharide from Enterococcus faecium WEFA23 was isolated and purified which includes four fractions, i.e. A23-1, A23-2, A23-3 and A23-4. • A23-2 was heteropolysaccharide composed of mannose, glucose and galactose, while A23-4 was homopolysaccharide consisted of mannose. • Both A23-2 and A23-4 exhibited free radicals scavenging capacities in vitro, and A23-2 showed higher antioxidant activity than A23-4. • A23-2 and A23-4 exhibited strong inhibition on adhesion of L. monocytogenes CMCC54007 to HT-29 cells. [ABSTRACT FROM AUTHOR]
- Subjects :
- *MICROBIAL exopolysaccharides
*ENTEROCOCCUS faecium
*MOLECULAR weights
*INFANTS
Subjects
Details
- Language :
- English
- ISSN :
- 01418130
- Volume :
- 128
- Database :
- Academic Search Index
- Journal :
- International Journal of Biological Macromolecules
- Publication Type :
- Academic Journal
- Accession number :
- 135198207
- Full Text :
- https://doi.org/10.1016/j.ijbiomac.2018.12.245