Back to Search
Start Over
Positive Correlation between Activated CypA/CD147 Signaling and MMP-9 Expression in Mice Inflammatory Periapical Lesion.
- Source :
-
BioMed Research International . 3/5/2019, p1-10. 10p. - Publication Year :
- 2019
-
Abstract
- Aim. Cyclophilin A (CypA)/CD147 signaling plays critical roles in the regulation of inflammation and bone metabolism. This study aimed to investigate the participation of CypA/CD147 in mice periapical lesions progression and its relationship with bone resorption. Methodology. Periapical lesions were induced by pulp exposure in the first lower molars of 40 C57BL/6J mice. The mice were sacrificed on days 0, 7, 14, 21, 28, 35, 42, and 49. Mandibles were harvested for X-ray imaging, microcomputed tomography scanning, histologic observation, immunohistochemistry, enzyme histochemistry, and double immunofluorescence analysis. Western blot was employed to further detect the related molecular signaling pathways in LPS-stimulated RAW 264.7 cells treated with CypA inhibitor. Results. The volume and area of the periapical lesions increased from day 0 to day 35 and remained comparably stable until day 49. Immunohistochemistry demonstrated that the CypA expression levels also increased from day 0 to day 35 and decreased until day 49, similar to CD147 expression (R2 = 0.4423, P < 0.05), osteoclast number (R2 = 0.5101, P < 0.01), and the expression of osteoclastogenesis-related matrix metalloproteinase 9 (MMP-9) (R2 = 0.4715, P < 0.05). Serial sections further confirmed the colocalization of CypA and CD147 on osteoclasts with immunohistochemistry. And the distribution of CypA-positive or CD147-positive cells was positively correlated with the dynamics of MMP-9-positive cells by using immunofluorescence analysis. Furthermore, CD147 and MMP-9 expression in RAW 264.7 cells were both downregulated with CypA inhibitor treatment (P < 0.05). Conclusions. The present study reveals the positive correlation of CypA/CD147 signaling and osteoclast-related MMP-9 expression in mice inflammatory periapical lesions progression. Therefore, intervention of CypA/CD147 signaling could probably provide a potential therapeutic target for attenuating inflammatory bone resorption. [ABSTRACT FROM AUTHOR]
- Subjects :
- *ANIMAL experimentation
*BONE resorption
*BONE growth
*CELL receptors
*CELLULAR signal transduction
*COMPUTED tomography
*CYTOKINES
*FLUORESCENT antibody technique
*GENE expression
*HISTOLOGICAL techniques
*IMMUNOHISTOCHEMISTRY
*INFLAMMATION
*MACROPHAGES
*MANDIBLE
*MICE
*MOLARS
*PERIAPICAL diseases
*WESTERN immunoblotting
*X-rays
*DISEASE progression
*LIPOPOLYSACCHARIDES
*MATRIX metalloproteinases
Subjects
Details
- Language :
- English
- ISSN :
- 23146133
- Database :
- Academic Search Index
- Journal :
- BioMed Research International
- Publication Type :
- Academic Journal
- Accession number :
- 135070348
- Full Text :
- https://doi.org/10.1155/2019/8528719