Preparation and application of a chemically modified laccase and copper phosphate hybrid flower-like biocatalyst.

Graphical abstract Highlights • hNFs biocatalyst was prepared by immobilizing modified laccase. • hNFs biocatalyst activity increased to 165% compared to laccase. • hNFs biocatalyst achieved 375% pH stability and 430% temperature stability. • hNFs biocatalyst catalyzed resveratrol to obtain 45% yield of dimer with no byproduct. • hNFs biocatalyst has higher catalytic yields, specificity than chemical catalyst. Abstract In order to face the increasing demand of laccase with high stability and high performance, laccase was chemically modified by acid anhydride, and copper ion (Ⅱ) was coordinated with the hetero atom of the modified laccase to obtain the nanoflower biocatalyst. The effects of different reaction time, laccase concentration and copper sulfate concentration on the formation of nanoflowers were investigated in detail. Compared to free laccase, the enzyme activity, pH stability and thermal stability of nanoflowers increased by 165%, 375% and 430%, respectively. Furthermore, the catalytic properties of our nanoflower biocatalysts were investigated by catalyzing the synthesis of viniferin. The results showed that a higher yield of 45.0% was obtained in shorter period of time than that of the free laccase (21.6%), and the product was single without other by-products. What's more, nanoflower biocatalyst exhibited excellent storage stability, repeatability and reproducibility in cycle operation. We believed that this study provided a feasible and efficient biocatalytic method for conversion of resveratrol to viniferin. These preliminary results indicated that nanoflowers would have great potential for application in biotransformation and biocatalysis. [ABSTRACT FROM AUTHOR]