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Preparation and Properties of 3-Halopyridine--adenine Dinucleotides, NAD +Analogues of Model Compounds.
- Source :
-
European Journal of Biochemistry . May76 Part 1, Vol. 64 Issue 2, p351-360. 10p. - Publication Year :
- 1976
-
Abstract
- The preparation of model compounds 1-(2′,6′-dichlorobenzyl)-3-halogenopyridinium and the study of their properties were achieved. Their chemical reduction to the corresponding 1,4-dihydropyridines is proved by spectroscopic analysis. 3-Iodopyridine—adenine dinucleotide was prepared by enzymic transglycosidation while the 3-chloro, 3-bromo and 3-iodo pyridine—adenine dinucleotides were synthesized from 3-amino-pyridine-adenine dinucleotide. The 3-halogenopyridine-adenine dinucleotides were proved to be active as hydrogen acceptors with alcohol as a substrate. The absorption band at 290 nm of cinnamaldehyde appeared to be a very sensitive tool for studying the enzymic reaction. With the alcohol dehydrogenase from yeast, only slight activity was detected. 3-Halogenopyridine—adenine dinucleotides are competitive inhibitors with respect to nicotinamide—adenine dinucleotide with alcohol dehydrogenase from yeast, lactate dehydrogenase and malate dehydrogenase. The use of 3-iodopyridine—adenine dinucleotide as a heavy-atom derivative for X-ray structure determination is proposed. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00142956
- Volume :
- 64
- Issue :
- 2
- Database :
- Academic Search Index
- Journal :
- European Journal of Biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 13488257
- Full Text :
- https://doi.org/10.1111/j.1432-1033.1976.tb10308.x