激活 FXR 抑制结肠癌细胞浸润转移及 MMP-7 的表达.

Objective: To investigate the mechanism of the farnesoid X receptor (FXR) activation by GW4064 to inhibit the growth of colon cancer cells. Methods: The effects of specific FXR agonist (GW4064) on the growth of HT-29 cells were studied in vitro by MTT. The infiltration and metastasis of HT-29 cells were detected by the transwell inserts. The mRNA expression of FXR and MMP-7 were determined by RT-PCR, The protein expression of FXR and MMP-7 were measured byWestern blot. Results: MTT results showed that the growth inhibition rate of GW4064 in human colorectal HT-29 cells was concentration dependent; Transwell inserts results showed that GW4064 inhibited the invasion and metastasis of colon cancer cells. Compared with the control group, the difference was statistically significant (P<0.05). RT-PCR and Western blot display GW4064 promoted FXR mRNA and protein expression and inhibited the expression of MMP-7. The expression of mRNA and protein was significantly different from that of the control group (P<0.05). Conclusion: GW4064 inhibited metastasis of colon cancer cells. The expression of mRNA and protein of FXR was up-regulated by FXR specific agonist in cell line HT-29, and MMP-7 is just opposite. The activation of FXR may inhibit the metastasis of colon cancer cells by inhibiting MMP-7. [ABSTRACT FROM AUTHOR]