A photoacoustic and fluorescence dual-mode probe for LTA4H imaging reveals inflammation site in murine.

Highlights • A photoacoustic and fluorescence dual-mode probe for LTA 4 H sensing was developed. • Cy-ASP has high selectivity and high sensitivity toward LTA 4 H. • Combined with PA technology, Cy-ASP could image the activity of LTA 4 H in mice without dissection. • The probe Cy-ASP could fast locate the inflammation site in mice. Abstract Inflammation widely occurs in various tissues and organs in the human body. Rapid and accurate diagnosis of inflammation is essential to avoid severe complications. This study aims to obtain a non-destructive, fast, and comprehensive tool for the detection of pathogenic sites of inflammation in vivo. A fluorescence and photoacoustic dual-mode imaging probe, Cy-ASP, was first developed to detect inflammatory biomarker LTA 4 H with good sensitivity and selectivity in living small animal. Furthermore, the relationship between LTA 4 H and NF-κB in an LPS-induced cell was investigated. This work provides a new idea for the clinical diagnosis of pneumonia and hepatitis and revealing the upstream regulatory mechanism of LTA 4 H in the pneumonia model. [ABSTRACT FROM AUTHOR]