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Transcriptome Analysis of Improved Wool Production in Skin-Specific Transgenic Sheep Overexpressing Ovine β-Catenin.
- Source :
-
International Journal of Molecular Sciences . Feb2019, Vol. 20 Issue 3, p620. 1p. 3 Diagrams, 3 Charts, 6 Graphs. - Publication Year :
- 2019
-
Abstract
- β-Catenin is an evolutionarily conserved molecule in the canonical Wnt signaling pathway, which controls decisive steps in embryogenesis and functions as a crucial effector in the development of hair follicles. However, the molecular mechanisms underlying wool production have not been fully elucidated. In this study, we investigated the effects of ovine β-catenin on wool follicles of transgenic sheep produced by pronuclear microinjection with a skin-specific promoter of human keratin14 (k14). Both polymerase chain reaction and Southern blot analysis showed that the sheep carried the ovine β-catenin gene and that the β-catenin gene could be stably inherited. To study the molecular responses to high expression of β-catenin, high-throughput RNA-seq technology was employed using three transgenic sheep and their wild-type siblings. These findings suggest that β-catenin normally plays an important role in wool follicle development by activating the downstream genes of the Wnt pathway and enhancing the expression of keratin protein genes and keratin-associated protein genes. [ABSTRACT FROM AUTHOR]
- Subjects :
- *CATENINS
*EMBRYOLOGY
*KERATIN
*TRANSGENIC animals
*WOOL
Subjects
Details
- Language :
- English
- ISSN :
- 16616596
- Volume :
- 20
- Issue :
- 3
- Database :
- Academic Search Index
- Journal :
- International Journal of Molecular Sciences
- Publication Type :
- Academic Journal
- Accession number :
- 134800876
- Full Text :
- https://doi.org/10.3390/ijms20030620