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Studies on the Mechanism and Stereospecificity of the Urocanase Reaction.
- Source :
-
European Journal of Biochemistry . 1971, Vol. 23 Issue 1, p198-202. 5p. - Publication Year :
- 1971
-
Abstract
- 1. Nuclear-magnetic-resonance spectroscopy was used to follow the course of the urocanase reaction. It was shown that 3-(4'-hydroxyimidazol-5'-yl)-propionate is the true enzyme product and that it spontaneously tautomerises to racemic 3-(imidazol-4'-one-5'-yl)-propionate. 2. Conversion of urocanate in deuterium oxide by a crude-enzyme preparation from Pseudomonas putida led to a product containing two adjacent CH²H-groups. Oxidative degradation of this product afforded optically pure (RR)-[²H2]succinic acid. This fining defines the stereochemistry of proton addition to the double bond of urocanate. [ABSTRACT FROM AUTHOR]
- Subjects :
- *RESONANCE
*SPECTRUM analysis
*DEUTERIUM
*OXIDES
*BIOCHEMISTRY
Subjects
Details
- Language :
- English
- ISSN :
- 00142956
- Volume :
- 23
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- European Journal of Biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 13476881
- Full Text :
- https://doi.org/10.1111/j.1432-1033.1971.tb01609.x