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Diurnal variation of metabolites in three individual participants.

Authors :
Gu, Fangyi
Klerman, Elizabeth B.
Kim, Sungduk
Moore, Steve
Yu, Kai
Albert, Paul S.
Caporaso, Neil E
Source :
Chronobiology International: The Journal of Biological & Medical Rhythm Research. Mar2019, Vol. 36 Issue 3, p332-342. 11p. 4 Charts, 3 Graphs.
Publication Year :
2019

Abstract

The circadian system influences virtually all biological functions. Understanding the impact of circadian variation on metabolism may provide insight into mechanisms of circadian-associated disorders and guide the implementation of chrono-therapy. Previous research has reported circadian variation in 7-20% of metabolites in human blood. In this study, untargeted metabolomics profiles were measured using blood of two healthy men and one healthy woman, collected every 2 h for up to 48 h under carefully controlled conditions. The pattern of variation of each metabolite over time was examined on each participant separately, using both one- and two-order harmonic models. A total of 100 of 663 metabolites, representing all metabolite categories, showed diurnal rhythmic concentrations that exceeded the Bonferroni threshold (P < 2.5 × 10−5). Overall, peak times of many metabolites were clustered during the afternoon-midnight, including the majority of amino acids, all peptides, all lysolipids and all phospholipids, whereas the majority of steroids peaked in the morning. We observed substantial inter-individual variation for both peak times and amplitudes in these three subjects. In conclusion, at least 15% of blood metabolites, representing a broad group of biological pathways, exhibit diurnal variation in three participants. The average peak times of most of these metabolites are clustered in morning or afternoon-midnight. Further work is needed to validate and extend this work in more individuals. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
07420528
Volume :
36
Issue :
3
Database :
Academic Search Index
Journal :
Chronobiology International: The Journal of Biological & Medical Rhythm Research
Publication Type :
Academic Journal
Accession number :
134749282
Full Text :
https://doi.org/10.1080/07420528.2018.1541901