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Orthognathic surgery induces genomewide changes longitudinally in DNA methylation in saliva.
- Source :
-
Oral Diseases . Mar2019, Vol. 25 Issue 2, p508-514. 7p. 2 Diagrams, 1 Chart, 1 Graph. - Publication Year :
- 2019
-
Abstract
- Objective: Orthognathic surgery dramatically changes morphology of the maxillofacial deformity and improves the malocclusion morphologically and functionally. We investigated the influence of orthognathic surgery on genomewide DNA methylation in saliva. Methods: Saliva was obtained from nine patients undergoing orthognathic surgery and two healthy reference individuals before and 3 months after orthognathic surgery. Genomewide DNA methylation profiling of saliva (341,482 CpG dinucleotides) was conducted using Infinium HumanMethylation450 BeadChips. Results: Comparison between pre‐ and postsurgery saliva samples revealed significant changes in DNA methylation patterns at 2,381 CpG sites (p < 0.01) with suggestive significance. The differentially methylated probe sets were significantly associated with the cancer pathway (p = 2.8 × 10−7; a false discovery rate q‐value = 3.7 × 10−4) and PI3K‐Akt signalling pathway (p = 2.4 × 10−5; a false discovery rate q‐value = 3.1 × 10−2). Conclusion: Pathway enrichment analysis of genes with suggestive significance demonstrated that altered DNA methylation in saliva of patients undergoing orthognathic surgery, possibly as a response to surgical stress or bone injury. Further studies with a large sample size and long‐term observation are needed to validate the phenomena identified in this study. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 1354523X
- Volume :
- 25
- Issue :
- 2
- Database :
- Academic Search Index
- Journal :
- Oral Diseases
- Publication Type :
- Academic Journal
- Accession number :
- 134737132
- Full Text :
- https://doi.org/10.1111/odi.12998