An airway organoid is forever.

In the last decade, the generation and maintenance of organotypic structures has been propelled to the center stage of biomedical research. In the lung, a variety of protocols has been devised to generate organoids mimicking lung structures, but most methods with human cells have complicated lengthy protocols or a progressive decline in differentiation potential and physiological function with increasing passaging. A new study from Sachs et al () seeks to solve these issues, providing a versatile methodology to efficiently isolate, indefinitely culture, and manipulate human airway organoids, potentially allowing the in vitro modeling of a plethora of lung diseases. A new methods resource reports long‐term propagation of human lung epithelium as three dimensional organoids which can be exploited for disease modeling. [ABSTRACT FROM AUTHOR]