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High-yield production of L-serine from glycerol by engineered Escherichia coli.
- Source :
-
Journal of Industrial Microbiology & Biotechnology . Feb2019, Vol. 46 Issue 2, p221-230. 10p. 1 Diagram, 3 Charts, 6 Graphs. - Publication Year :
- 2019
-
Abstract
- L-Serine is widely used in pharmaceutical, food and cosmetic industries, and the direct fermentation to produce L-serine from cheap carbon sources such as glycerol is greatly desired. The production of L-serine by engineered Escherichia coli from glycerol has not been achieved so far. In this study, E. coli was engineered to efficiently produce L-serine from glycerol. To this end, three L-serine deaminase genes were deleted in turn, and all of the deletions caused the maximal accumulation of L-serine at 0.06 g/L. Furthermore, removal of feedback inhibition by L-serine resulted in a titer of 1.1 g/L. Additionally, adaptive laboratory evolution was employed to improve glycerol utilization in combination with the overexpression of the cysteine/acetyl serine transporter gene eamA, leading to 2.36 g/L L-serine (23.6% of the theoretical yield). In 5-L bioreactor, L-serine titer could reach up to 7.53 g/L from glycerol, demonstrating the potential of the established strain and bioprocess. [ABSTRACT FROM AUTHOR]
- Subjects :
- *SERINE
*GLYCERIN
*ESCHERICHIA coli
*DEAMINASES
*CYSTEINE
Subjects
Details
- Language :
- English
- ISSN :
- 13675435
- Volume :
- 46
- Issue :
- 2
- Database :
- Academic Search Index
- Journal :
- Journal of Industrial Microbiology & Biotechnology
- Publication Type :
- Academic Journal
- Accession number :
- 134563651
- Full Text :
- https://doi.org/10.1007/s10295-018-2113-6