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LncRNA DANCR promotes cervical cancer progression by upregulating ROCK1 via sponging miR‐335‐5p.

LncRNA DANCR promotes cervical cancer progression by upregulating ROCK1 via sponging miR‐335‐5p.

Authors :
Liang, Huiling
Zhang, Chunyan
Guan, Hongya
Liu, Jia
Cui, Yuanbo
Source :
Journal of Cellular Physiology. May2019, Vol. 234 Issue 5, p7266-7278. 13p.
Publication Year :
2019

Abstract

Emerging evidence highlights the key regulatory roles of long noncoding RNAs (lncRNAs) in the initiation and progression of numerous malignancies. The lncRNA identified as differentiation antagonizing nonprotein coding RNA (DANCR) is a novel lncRNA widely involved in the development of multiple human cancers. However, the function of DANCR and its potential molecular mechanism in cervical cancer remain unclear. In this study, we discovered that DANCR was significantly elevated in cervical cancer tissues and cells, and was closely correlated with poor prognosis of cervical cancer patients. In addition, knockdown of DANCR inhibited proliferation, migration, and invasion of cervical cancer cells in vitro, indicating that DANCR functioned as an oncogene in cervical cancer. Moreover, we verified that DANCR could directly bind to miR‐335‐5p, isolating miR‐335‐5p from its target gene Rho‐associated coiled‐coil containing protein kinase 1 (ROCK1). Functional analysis showed that DANCR regulated ROCK1 expression by competitively binding to miR‐335‐5p. Further cellular behavioral experiments revealed that miR‐335‐5p mimics and ROCK1 knockdown reversed the effects of upregulated DANCR on proliferation, migration, invasion, and epithelial‐mesenchymal transition (EMT) of cervical cancer cells by rescue assays. In summary, this study demonstrated that DANCR promoted cervical cancer progression by functioning as a competing endogenous RNA (ceRNA) to regulate ROCK1 expression via sponging miR‐335‐5p, suggesting a novel potential therapeutic target for cervical cancer. Long noncoding RNAs (lncRNA) differentiation antagonizing nonprotein coding RNA (DANCR) was significantly elevated in cervical cancer tissues and closely correlated with poor prognosis in cervical cancer patients. LncRNA DANCR promoted cervical cancer progression by functioning as a competing endogenous RNA (ceRNA) to regulate Rho‐associated coiled‐coil containing protein kinase 1 (ROCK1) expression via sponging miR‐335‐5p in vitro. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
00219541
Volume :
234
Issue :
5
Database :
Academic Search Index
Journal :
Journal of Cellular Physiology
Publication Type :
Academic Journal
Accession number :
134233756
Full Text :
https://doi.org/10.1002/jcp.27484